Unpublished pilot study (with Judith Mantell and Paul Verkade) : The HeLa cell is plung frozen and imaged A) Optically for 10nm gold/488/EGF or 5nm gold/635/Tfn at -196C or B)Low Magnification Cryo-TEM or C) High magnification cryo-TEM.
This is only possible where it is thin ice, like the edges of cells. Note the unbound untaken up gold in C visible on the surface. Tomography next time!
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