Unpublished pilot study (with Judith Mantell and Paul Verkade) :  The HeLa cell is plung frozen and imaged  A) Optically for 10nm gold/488/EGF or 5nm gold/635/Tfn at -196C or B)Low Magnification Cryo-TEM or C) High magnification cryo-TEM.
This is only possible where it is thin ice, like the edges of cells. Note the unbound untaken up gold in C visible on the surface. Tomography next time!
Click here for main page